ABSTRACT

Aim: To access the anticoagulant effect of n-hexane (HE), chloroform (CE) and distilled water (DE) extracts ofA. sarcocolla.

Methodology: Resin of A. sarcocolla was extracted using n-hexane, choloform and distilled water using maceration. The extract was concentrated using rotary evaporator. Four different doses of extract (2.5, 5, 7.5 and 10 µg) were prepared for performing assay. In-vitro anticoagulant effects using human blood were studied in 42 healthy males age between 25-35 years.

Results: All extracts significantly increased the PT in comparison to control. At highest dose of 10µg, with the exception of distilled water extract, all extracts demonstrated highly significant (p<0.001) increase in APTT.

Conclusion: The data of the present study demonstrates that extracts ofA. sarcocolla possess anticoagulant activity.

Keywords: Anticoagulant; Astragalussarcocolla; Prothrombin time; Activated Partial Thromboplastin time