In-vitro anticoagulant activity of three extracts of Astragalussarcocolla
Muhammad UsamaMazhar, KaenaatMuneer; Fareeha Anwar, Waqar Ahmed Siddiqui, A Q Qureshi, R Bilal, B Ahmad
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ABSTRACT
Aim: To access the anticoagulant effect of
n-hexane (HE), chloroform (CE) and distilled water (DE) extracts ofA.
sarcocolla.
Methodology: Resin of A. sarcocolla was extracted using n-hexane, choloform
and distilled water using maceration. The extract was concentrated using rotary
evaporator. Four different doses of extract (2.5, 5, 7.5 and 10 µg) were
prepared for performing assay. In-vitro anticoagulant effects using human blood
were studied in 42 healthy males age between 25-35 years.
Results: All
extracts significantly increased the PT in comparison to control. At highest dose
of 10µg, with the exception of distilled water extract, all extracts
demonstrated highly significant (p<0.001) increase in APTT.
Conclusion: The data of the present study demonstrates
that extracts ofA. sarcocolla possess anticoagulant activity.
Keywords: Anticoagulant; Astragalussarcocolla; Prothrombin
time; Activated
Partial Thromboplastin time
ABSTRACT
Aim: To access the anticoagulant effect of
n-hexane (HE), chloroform (CE) and distilled water (DE) extracts ofA.
sarcocolla.
Methodology: Resin of A. sarcocolla was extracted using n-hexane, choloform
and distilled water using maceration. The extract was concentrated using rotary
evaporator. Four different doses of extract (2.5, 5, 7.5 and 10 µg) were
prepared for performing assay. In-vitro anticoagulant effects using human blood
were studied in 42 healthy males age between 25-35 years.
Results: All
extracts significantly increased the PT in comparison to control. At highest dose
of 10µg, with the exception of distilled water extract, all extracts
demonstrated highly significant (p<0.001) increase in APTT.
Conclusion: The data of the present study demonstrates
that extracts ofA. sarcocolla possess anticoagulant activity.
Keywords: Anticoagulant; Astragalussarcocolla; Prothrombin
time; Activated
Partial Thromboplastin time