ABSTRACT

Background: Gayam seed is one of the native plan of Indonesia, has a high amylose content and is a good source of resistant starch (RS). The autoclaving-cooling cycle treatment is the physical modification to increase RS of starch. Resistant starch has a positive impact on health, such as preventing the increase in blood glucose levels significantly. The high RS in gayam starch is needed for prevention of diabetes mellitus.

Aim: To evaluate the antidiabetic properties (blood glucose and short chain fatty acids/SCFA levels) of modified gayamstarch in vivo.

Methods: The modified gayam starch were prepared by autoclaving-cooling 3 cycles. In vivo studies was prepared as isocaloric feed for three groups of diabetic Sprague Dawley rats induced streptozotocin-nicotinamide (STZ-NA) i.e (a) standar diet AIN 93 M (DM), (b) native gayam starch diet (NGS), and (c) modified gayam starch by autoclaving-cooling for three cycles diet (MGS).evaluation the in vivo effects on blood glucose and SCFA levels.

Results:In vivo studies in 28 days experiments showed that the intervention of modified gayam starch decreased blood glucose and increased SCFA content, were more significant in MGS than NGS diet.Conclusion: It was concluded that the intervention of modified gayam starch decreased blood glucose and increased SCFA content.

Keywords: gayam, modified starch, autoclaving-cooling cycles, antidiabetic, in vivo