The Effect of Titanium Dioxide Nanoparticle on some properties of Resistance Gram Negative Bacteria
Payman A. Hamasaeed
1192
ABSTRACT
In this study,
the bacteriostatic,bactericidal,
ant-biofilm, and molecular effectsof titanium dioxide nanoparticles (TiO2NP)against
multidrug resistance (MDR),Extended Spectrum βeta-Lactamase (ESBL)andAmpC
producer pathogenic isolates from different clinical specimens was assessed. Based on the results and
given the(Minimum Inhibitory Concentration), MBC (Minimum Bactericidal
Concentration), of titanium dioxide (10 nm size) were 0.488 μg/ml and 0.97
μg/ml and for Tio2NP (100 nm size) were 7.90μg/ml and 15.80for resistance
pathogenicE.coli isolates.On the other hand, the inhibition zoneof incubated
isolates in 10nm and 100 nm nanoparticles increased non significantly as
compared with control and small size was more effective than large size, and
there is no resistance against nanoparticles in drugs resistance pathogenic
bacteriaWe showed that bacterial biofilm growth was reduced at
MICconcentrations of TiO2NPs compared with another test without the NPs.
FurthermorePolymerase chain reaction amplification
of AmpCscreened and sequence alignments, analyses performed using the
Basic Local Alignment Search Tool (BLAST) online program that compare the
results of traces nitrogen bases of the gene within the National Center for
Biotechnology Information (NCBI) the result showed high similarities (98%)with
the AmpC for the E coli isolate (control), This study provides that substitution
in the nitrogen basethymine to guanine in sequence 17 and guanine to adenine in
sequence 96 and 113was detected after incubation in titanium dioxide 10nm and substitution
guanine to adenine in nitrogen base 92 and cytosine to thymine in nitrogen base
184 after incubation in Tio2NP 100nmConclusion:
Antibiotics resistance beta-lactam gram-negative bacteria isolated from
clinical samples has been detectedin hospitals. Since this bacterium is highly
resistant to drugs, several new methods have been investigated to fight against
it. Applying nanoparticles of titanium dioxide
as a strongantimicrobialcompound,is one of the most
effective ways to solve the bacterialmultidrug resistance increase problem.
Keywords:MDR,AmpC, Gram negative bacteria,Titanium
dioxide nanoparticle
ABSTRACT
In this study,
the bacteriostatic,bactericidal,
ant-biofilm, and molecular effectsof titanium dioxide nanoparticles (TiO2NP)against
multidrug resistance (MDR),Extended Spectrum βeta-Lactamase (ESBL)andAmpC
producer pathogenic isolates from different clinical specimens was assessed. Based on the results and
given the(Minimum Inhibitory Concentration), MBC (Minimum Bactericidal
Concentration), of titanium dioxide (10 nm size) were 0.488 μg/ml and 0.97
μg/ml and for Tio2NP (100 nm size) were 7.90μg/ml and 15.80for resistance
pathogenicE.coli isolates.On the other hand, the inhibition zoneof incubated
isolates in 10nm and 100 nm nanoparticles increased non significantly as
compared with control and small size was more effective than large size, and
there is no resistance against nanoparticles in drugs resistance pathogenic
bacteriaWe showed that bacterial biofilm growth was reduced at
MICconcentrations of TiO2NPs compared with another test without the NPs.
FurthermorePolymerase chain reaction amplification
of AmpCscreened and sequence alignments, analyses performed using the
Basic Local Alignment Search Tool (BLAST) online program that compare the
results of traces nitrogen bases of the gene within the National Center for
Biotechnology Information (NCBI) the result showed high similarities (98%)with
the AmpC for the E coli isolate (control), This study provides that substitution
in the nitrogen basethymine to guanine in sequence 17 and guanine to adenine in
sequence 96 and 113was detected after incubation in titanium dioxide 10nm and substitution
guanine to adenine in nitrogen base 92 and cytosine to thymine in nitrogen base
184 after incubation in Tio2NP 100nmConclusion:
Antibiotics resistance beta-lactam gram-negative bacteria isolated from
clinical samples has been detectedin hospitals. Since this bacterium is highly
resistant to drugs, several new methods have been investigated to fight against
it. Applying nanoparticles of titanium dioxide
as a strongantimicrobialcompound,is one of the most
effective ways to solve the bacterialmultidrug resistance increase problem.
Keywords:MDR,AmpC, Gram negative bacteria,Titanium
dioxide nanoparticle