Taha M. Maaroof, Ava Taher. Ismael, Jalal Ali. Jalal, Rafal A. Al-Rawi

P53 Gene and Ki 67 Proliferative Index in Colorectal Adenomas- A Clinico-pathological Study

Taha M. Maaroof, Ava Taher. Ismael, Jalal Ali. Jalal, Rafal A. Al-Rawi



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ABSTRACT
Aim: To study p53gene expression and Ki-67 proliferative index in colorectal adenomas and its relation to variable clinicopathological parameters.
Method: A retrospective study of 100 formalin-fixed and paraffin-embedded samples from 84 patients with adenomatous polyps was selected from the files of the histopathological department at Rizgary Teaching Hospital and some of the private histopathological laboratories in Erbil city during the period from January 2014-January 2018 who underwent elective colonoscopy and subsequently polyp removal. P53 and Ki-67 expression were studied by immunohistochemistry using the monoclonal mouse anti-human p53 protein (clone DO-7)and monoclonal mouse anti-humanantibody (Clone MIB-1) respectively, by the standard streptavidin-biotin immunoperoxidase method. The expression of p53 and ki67were correlated with the clinicopathologic parametersand statistically analyzed by SPSS version 22.
Results: Overexpression of p53 and Ki-67 were seen in 94% and 79% of cases respectively. A strong significant relationship was observed between p53 expression and degree of dysplasia (p=0.004), advanced adenomas (p=0.001) and size of the adenomas (p=0.001). A significant relationship was observed between Ki-67PI and the gender of the patients (p=0.03). There is a strong significant statistical relationship between the type of adenomas and the degree of dysplasia (p= 0.005). There isa strong significant statistical relationship between p53 expression and Ki-67 PI (p=0.001). There was immune-expression of p53 and Ki-67 and clinicopathological parameter differences between synchronous adenomas from the same patients.
Conclusion: P53 expression is a frequent finding in large-sized, high-grade dysplasia and advanced adenoma. The Ki-67 expression in relation to clinicopathological parameters was significant in relation to the sex, and Ki-67 increases with size, type of adenoma, high-grade dysplasia and advanced adenomas. The high-grade dysplasia is seen more in villous adenomas. Increasing p53expression in colorectal adenomas is correlated with an increasing ki-67 activity index.
Key words: Colorectal Adenoma, P53, Ki-67

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