ABSTRACT

Aim: To study the frequency distribution of alleles and genotypes and to identify the association of polymorphic markers I/DACE gene and M235TAGT gene with hypertension in patients with type 2diabetes mellitus (DM)in the Russian population.

Methods: 45 patients with type 2 DM and hypertension 2-3 stage were examined. The study of carbohydrate, lipid metabolism, morning albuminuria,24h-blood pressure monitoringweredone. Identification of polymorphic markers I/DACE gene and M235TAGT gene was performed using PCR-based assay.

Results: the evaluation of laboratory parameters in patients with type 2DM and hypertension revealed the excess of the target values of carbohydrate and lipid metabolism. When analyzing the frequency distribution of alleles and genotypes of the ACE gene, the ratio of pathological DD genotypes in the patient group in comparison with the control group was: 40.0% and 14.29% (p=0.043); II genotypes - 17.78% and 28.57% (p=0.453); heterozygotes (IDgenotypes) -42.22% and 57.14% (p=0.348); allele D was identified in 61.11% and 42.86%, respectively (p=0.096).The results of AGT gene genotyping showed that the frequency of TT genotype in patients with DM type 2 and hypertension was 37.78%, heterozygotes (MT) - 62.23%, and MM genotype - 0%. In the control group, the MT genotype was identified in all subjects (100%).

Conclusion: presence of the DD genotype of the I/Dpolymorphism ACE gene and the TT genotype of the M235T polymorphism AGT gene are associated with the development of hypertension in patients with type 2diabetes mellitus.

Keywords: Diabetes mellitus, hypertension, angiotensin-converting enzyme, angiotensinogen, gene, poly