Effects of Progesterone and Estradiol on the Inflammatory and Apoptotic Markers of Ovariectomized rats Challenged with Acute Septic Systemic Inflammation
Sinan Subhi Farhan, Samir Saad Mahgoub, Saadabdulrahman Hussain, Rasha Abdulateef Abdul Hussein
1097
ABSTRACT
Background: The inflammatory responses
during septic systemic inflammation were affected by the differential role of
progesterone and estrogen that demonstrated pro-inflammatory and
anti-inflammatory roles.
Aim: The present study was designed to
evaluate the differential effects of estradioland progesterone supplementation
on the inflammatory and apoptotic responses in an ovariectomized rat model of
acute systemic septic inflammation (SSI).
Methods: The present study was conducted on 60
female Wistar rats. 40mg/kg estradiol and 5 mg/kg progesterone were given s.c.
to ovariectomized (OVX) rats after induction of systemic septic inflammation
(SSI) through caecum puncture with a 21-gauge needle.
Results: TNF-α, CRP, ALT, estradiol, and
progesterone were evaluated in sera; additionally, iNOS, COX-II, and caspase-3
were evacuated in liver tissues homogenates using ELISA method. In OVX rats
challenged with SSI, serum TNF-α, CRP and ALT levels were significantly
increased associated with a decrease in serum estradiol levels. They also
showed overexpression of the iNOSand increased activity of COX-II and caspase-3
in the livercompared to non-OVX rats subjected to SSI. Supplementation with
estradiol significantly decreases all serum and liver tissuemarkers of
inflammation and decreased apoptosis. In contrast, OVX rats supplemented with
progesterone,SSI resulted in a significant increase of the studied markers.
Conclusion: supplementation of
estradiolin OVX rats challenged with SSI significantly attenuated the systemic
andliver inflammatory and apoptotic markers. Meanwhile, supplementation with
progesterone exacerbates the effects of the inflammatory markers and increases
the tendency of apoptosis in the liver tissue.
Keywords: Septicsystemic inflammation,
liver, estradiol, progesterone, apoptosis
ABSTRACT
Background: The inflammatory responses
during septic systemic inflammation were affected by the differential role of
progesterone and estrogen that demonstrated pro-inflammatory and
anti-inflammatory roles.
Aim: The present study was designed to
evaluate the differential effects of estradioland progesterone supplementation
on the inflammatory and apoptotic responses in an ovariectomized rat model of
acute systemic septic inflammation (SSI).
Methods: The present study was conducted on 60
female Wistar rats. 40mg/kg estradiol and 5 mg/kg progesterone were given s.c.
to ovariectomized (OVX) rats after induction of systemic septic inflammation
(SSI) through caecum puncture with a 21-gauge needle.
Results: TNF-α, CRP, ALT, estradiol, and
progesterone were evaluated in sera; additionally, iNOS, COX-II, and caspase-3
were evacuated in liver tissues homogenates using ELISA method. In OVX rats
challenged with SSI, serum TNF-α, CRP and ALT levels were significantly
increased associated with a decrease in serum estradiol levels. They also
showed overexpression of the iNOSand increased activity of COX-II and caspase-3
in the livercompared to non-OVX rats subjected to SSI. Supplementation with
estradiol significantly decreases all serum and liver tissuemarkers of
inflammation and decreased apoptosis. In contrast, OVX rats supplemented with
progesterone,SSI resulted in a significant increase of the studied markers.
Conclusion: supplementation of
estradiolin OVX rats challenged with SSI significantly attenuated the systemic
andliver inflammatory and apoptotic markers. Meanwhile, supplementation with
progesterone exacerbates the effects of the inflammatory markers and increases
the tendency of apoptosis in the liver tissue.