Melatonin and Polymyxin B Administration, Alone or in Combination, Prevent the Elevation of Random Blood Glucose and Lactate Levels in Wistar Rats Endotoxicosis Model
Satrio Adi Wicaksono, Diana Kumalasari, Alice Valeria, Sulistiyati Bayu Utami
1082
ABSTRACT
Background: Sepsis induces
hyperglycemia due to insulin resistance and hyperlactatemia due to
anaerobic glycolysis pathway caused by tissue hypoxia. Melatonin is an antioxidant that may prevent increased glucose and increased lactate levels, however its role in sepsis need further investigation.
Aim:
To observe the effects of melatonin with and without Polymyxin B on random blood glucose and
lactate levels in Wistar rats
endotoxicosis model.
Methods: This was animal
experimental study with
randomized control design. There were twenty four male Wistar rats that were intraperitoneally injected
with 2
mg lipopolysaccharide (LPS) per 200 grams
rats and randomly
divided into four groups, i.e. control group receiving placebo, melatonin only group, Polymyxin B only group, and combination group
(receiving melatonin and Polymyxin B). The effects of oral melatonin and Polymyxin B treatments on random blood glucose and lactate levels
were investigated from the retro-orbital blood vessel sampling in baseline (B), 6 hours
after intraperitoneally LPS injection
(T0), 1 hour (T1) and 2 hours after
melatonin administration (T2).
The means of
glucose and lactate levels were analyzed with Student's t-test using computer
program.
Results:
There
were no significant differences
in random blood glucose and lactate levels between
control group, melatonin only group, Polymyxin
B only group and combination group in B,
T0, and
T1. In T2, there were significantly lower
levels of random blood glucose in melatonin only group (132.83 ± 10.7 mg/dL),
in Polymyxin B only group (131.06 ± 11.53 mg/dL), and in combination group
(130.03 ± 11.06 mg/dL) in comparison with control group (171.67 ± 15.88 mg/dL)
(p<0.05). In T2, there were significantly lower levels of lactate in
Polymyxin B only group (11.6 ± 1.5 mmol/L), and in combination group (10.0 ± 1.1
mmol/L) in comparison with control group (16.9 ± 1.2 mmol/L) (p<0.05). There
was also lower levels of lactate in melatonin only group (11.6 ± 6.6 mmol/L)
compared to control group, although it was not statistically significant (p>0.05).
Conclusion:
Melatonin administration as well Polymyxin
B, alone or in combination, could prevent the elevation of random blood glucose
and lactate levels in Wistar rats endotoxicosis model. Therefore, if confirmed by further research,
melatonin might have a role in management of endotoxicosis.
Keywords: endotoxicosis,
random blood glucose, lactate, melatonin,
lipopolysaccharide
ABSTRACT
Background: Sepsis induces
hyperglycemia due to insulin resistance and hyperlactatemia due to
anaerobic glycolysis pathway caused by tissue hypoxia. Melatonin is an antioxidant that may prevent increased glucose and increased lactate levels, however its role in sepsis need further investigation.
Aim:
To observe the effects of melatonin with and without Polymyxin B on random blood glucose and
lactate levels in Wistar rats
endotoxicosis model.
Methods: This was animal
experimental study with
randomized control design. There were twenty four male Wistar rats that were intraperitoneally injected
with 2
mg lipopolysaccharide (LPS) per 200 grams
rats and randomly
divided into four groups, i.e. control group receiving placebo, melatonin only group, Polymyxin B only group, and combination group
(receiving melatonin and Polymyxin B). The effects of oral melatonin and Polymyxin B treatments on random blood glucose and lactate levels
were investigated from the retro-orbital blood vessel sampling in baseline (B), 6 hours
after intraperitoneally LPS injection
(T0), 1 hour (T1) and 2 hours after
melatonin administration (T2).
The means of
glucose and lactate levels were analyzed with Student's t-test using computer
program.
Results:
There
were no significant differences
in random blood glucose and lactate levels between
control group, melatonin only group, Polymyxin
B only group and combination group in B,
T0, and
T1. In T2, there were significantly lower
levels of random blood glucose in melatonin only group (132.83 ± 10.7 mg/dL),
in Polymyxin B only group (131.06 ± 11.53 mg/dL), and in combination group
(130.03 ± 11.06 mg/dL) in comparison with control group (171.67 ± 15.88 mg/dL)
(p<0.05). In T2, there were significantly lower levels of lactate in
Polymyxin B only group (11.6 ± 1.5 mmol/L), and in combination group (10.0 ± 1.1
mmol/L) in comparison with control group (16.9 ± 1.2 mmol/L) (p<0.05). There
was also lower levels of lactate in melatonin only group (11.6 ± 6.6 mmol/L)
compared to control group, although it was not statistically significant (p>0.05).
Conclusion:
Melatonin administration as well Polymyxin
B, alone or in combination, could prevent the elevation of random blood glucose
and lactate levels in Wistar rats endotoxicosis model. Therefore, if confirmed by further research,
melatonin might have a role in management of endotoxicosis.
Keywords: endotoxicosis,
random blood glucose, lactate, melatonin,
lipopolysaccharide