Molecular Characterization of Extended spectrum beta-lactamases (ESBLs) Carbapenem-Resistant Genesin Klebsiella pneumoniae Isolated from Iranian Hospital
Sajad Yaghoubi, Shahriar Yavari, Zohreh Baseri,, Meghdad Abdollahpour-Alitappeh, Asiyeh Dezhkam, Yousef Erfani
1072
ABSTRACT
Background-Carbapenem-resistant Klebsiella isolates are describedas one of
the most significant problemsworldwide. The aim of this study was to determinethe
prevalence of extended spectrum beta-lactamase (ESBL)subtypes and carbapenem-resistant
genes inKlebsiellapneumoniae(K. pneumonia) speciesisolatedfrom
patients at Shariatee Hospital in Tehran, Iran.
Methods: Fifty isolates of K. pneumoniae were isolated and identified from
Shariatee Hospital in Tehran from January 2014 to February 2016. Disk diffusion
and E-test minimum inhibitory concentration (MIC) testing methods were carried
out to characterize the isolates.Modified Hodge Testing (MHT) and carbapenem
inactivation method test (CIM), was used to confirm carbapenemase activity.Finally,
Sequencing and polymerase chain reaction (PCR) was applied for further analysis.
Results: Our findings showed that the prevalence of blaCTX-M 33 (63.46%) genes
was the most prevalent ones. blaCTX-M15 19 (36.53%)was found to be
the most commonly detected subtype in CTX-M groups.blaKPC, blaOXA-48, and
blaNDM1 were detected in 3 (5.76%), 6 (11.53%), and 12 (23.7%) of the
carbapenem-resistant isolates, respectively.
Conclusions:
Our studydisplayed that the CIM test has a
great potential to detect carbapenem-resistant Klebsiella (CRK). Based
on our results,there are a need for further considerations regarding the
emergence anddiagnosis of isolates harboring ESBL subtypes and carbapenem genes.
Further attention should be paidfor the treatment of patients with highlyresistant-isolates.
Keywords: Klebsiella pneumoniae, New Delhi metallo-beta-lactamase-1,
Extended-spectrum ß-lactamase
ABSTRACT
Background-Carbapenem-resistant Klebsiella isolates are describedas one of
the most significant problemsworldwide. The aim of this study was to determinethe
prevalence of extended spectrum beta-lactamase (ESBL)subtypes and carbapenem-resistant
genes inKlebsiellapneumoniae(K. pneumonia) speciesisolatedfrom
patients at Shariatee Hospital in Tehran, Iran.
Methods: Fifty isolates of K. pneumoniae were isolated and identified from
Shariatee Hospital in Tehran from January 2014 to February 2016. Disk diffusion
and E-test minimum inhibitory concentration (MIC) testing methods were carried
out to characterize the isolates.Modified Hodge Testing (MHT) and carbapenem
inactivation method test (CIM), was used to confirm carbapenemase activity.Finally,
Sequencing and polymerase chain reaction (PCR) was applied for further analysis.
Results: Our findings showed that the prevalence of blaCTX-M 33 (63.46%) genes
was the most prevalent ones. blaCTX-M15 19 (36.53%)was found to be
the most commonly detected subtype in CTX-M groups.blaKPC, blaOXA-48, and
blaNDM1 were detected in 3 (5.76%), 6 (11.53%), and 12 (23.7%) of the
carbapenem-resistant isolates, respectively.
Conclusions:
Our studydisplayed that the CIM test has a
great potential to detect carbapenem-resistant Klebsiella (CRK). Based
on our results,there are a need for further considerations regarding the
emergence anddiagnosis of isolates harboring ESBL subtypes and carbapenem genes.
Further attention should be paidfor the treatment of patients with highlyresistant-isolates.
Keywords: Klebsiella pneumoniae, New Delhi metallo-beta-lactamase-1,
Extended-spectrum ß-lactamase