Biochemically and Genetically Evaluation of Antibiotic Resistance of E. coli Isolated from Egg Yolk Distributed in Guilan Province, Iran
Parvaneh Rahseparpour, Leila Modiri, Mirsasan Mirpour, Arash Chaichi Nosrati
1021
ABSTRACT
Aim: The use of antibiotics in poultry can lead to
the public health risks.In this study,
the antibiotic resistance of E. coli pathogens isolated from commercial
eggs distributed in Guilan province (Iran) is investigated.
Methodology: Sample of eggs’ shells are used for general
identification of E. coli. TwelveE. colipathogens are tested for
resistance to Chloramphenicol (CAP) and Fluorophenicol
(FP)antibiotics by the disk diffusion method on Mueller-Hinton agar plates.
Isolates that shows the highest degree of resistance are examined to detect of
antibiotic resistance genes, floR, cmlA and cat1 genes,
based on PCR method and gel electrophoresis.
Results: It can be observed that seven and ten E.
coli isolates demonstrate resistance to CAP and FPantibiotics, respectively,
based ondisk diffusion method. In contrast, seven and three E. coli
isolates demonstrate resistance gene to FP and CAP antibiotics. The results
show good correlation between antibiotic resistance phenotype and genotypes in
these E. coli isolates.
Conclusion: It seems we should set rules and provide
routine monitoring for testing and controlling the unnecessary and unconscious
use of antibiotics in the diet of chicken breeding.
Keyword: Antibiotic
resistance; E.
colipathogens; Egg yolk; Biochemistry; Genetic
ABSTRACT
Aim: The use of antibiotics in poultry can lead to
the public health risks.In this study,
the antibiotic resistance of E. coli pathogens isolated from commercial
eggs distributed in Guilan province (Iran) is investigated.
Methodology: Sample of eggs’ shells are used for general
identification of E. coli. TwelveE. colipathogens are tested for
resistance to Chloramphenicol (CAP) and Fluorophenicol
(FP)antibiotics by the disk diffusion method on Mueller-Hinton agar plates.
Isolates that shows the highest degree of resistance are examined to detect of
antibiotic resistance genes, floR, cmlA and cat1 genes,
based on PCR method and gel electrophoresis.
Results: It can be observed that seven and ten E.
coli isolates demonstrate resistance to CAP and FPantibiotics, respectively,
based ondisk diffusion method. In contrast, seven and three E. coli
isolates demonstrate resistance gene to FP and CAP antibiotics. The results
show good correlation between antibiotic resistance phenotype and genotypes in
these E. coli isolates.
Conclusion: It seems we should set rules and provide
routine monitoring for testing and controlling the unnecessary and unconscious
use of antibiotics in the diet of chicken breeding.
Keyword: Antibiotic
resistance; E.
colipathogens; Egg yolk; Biochemistry; Genetic