Molecular Detection of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa and Pseudomonas fluorescence Clinical Isolates in Khartoum State, Sudan
Najem Aldin M. Osman, Ammar M. Hashim, Nadir Abuzeid, Hisham Altayb, Muataz Mohmed Eldirdery, Ali Suleiman Elwakeel Ali Mohamed Elhassan EleragI
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ABSTRACT
Background: Antibiotics resistant including
carbapenems among pseudomonas species is one of the critical health problems
which lead to limited treatment options. Therefore, precise identification and
antibiotic testing is necessary for effectual treatment.
Aim: To determine Metallo-β-Lactamase (blaVIM
and blaIMP) producing pseudomonas species among
different Pseudomonal infections.
Methods: Standard microbiological methods,
antibiotics susceptibility tests and PCR were performed to identify
Metallo-β-Lactamase producing pseudomonas species
Results: A total of 40 Pseudomonas isolates were
recovered. Pseudomonas fluorescence was found to be 85% (n = 34)
while Pseudomonas aeruginosa was 15% (n = 6). All Ps. aeruginosa
isolates were IMP and VIM positive (100%), while just nine (22%) isolates of Ps.
fluorescence were bearing IMP and eight (20%) were VIM positive.
Conclusion: Our finding revealed a high frequency
of MBLs producing Pseudomonas species where Ps. fluorescens was more
frequently isolated.
Keywords: Carbapenems, Psaeruginosa, Ps.
fluorescence, IMP, VIM.
ABSTRACT
Background: Antibiotics resistant including
carbapenems among pseudomonas species is one of the critical health problems
which lead to limited treatment options. Therefore, precise identification and
antibiotic testing is necessary for effectual treatment.
Aim: To determine Metallo-β-Lactamase (blaVIM
and blaIMP) producing pseudomonas species among
different Pseudomonal infections.
Methods: Standard microbiological methods,
antibiotics susceptibility tests and PCR were performed to identify
Metallo-β-Lactamase producing pseudomonas species
Results: A total of 40 Pseudomonas isolates were
recovered. Pseudomonas fluorescence was found to be 85% (n = 34)
while Pseudomonas aeruginosa was 15% (n = 6). All Ps. aeruginosa
isolates were IMP and VIM positive (100%), while just nine (22%) isolates of Ps.
fluorescence were bearing IMP and eight (20%) were VIM positive.
Conclusion: Our finding revealed a high frequency
of MBLs producing Pseudomonas species where Ps. fluorescens was more
frequently isolated.
Keywords: Carbapenems, Psaeruginosa, Ps.
fluorescence, IMP, VIM.